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KMID : 1033620180450020082
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Clinical and Experimental Reproductive Medicine
2018 Volume.45 No. 2 p.82 ~ p.87
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Evaluation of prooxidant-antioxidant balance in in vitro fertilization-conceived mice
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Ahmadi Hamid
Fathi Fardin
Moeini Ashraf
Amidi Fardin
Sobhani Aligholi
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Abstract
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Objective: Concerns about the safety of assisted reproductive technology (ART) have been raised, as some studies have shown elevated incidence rates of childhood cancer, asthma, allergies, and other diseases in ART-conceived babies. Findings regarding the health of ART-conceived babies are controversial. The present study was conducted to evaluate the prooxidant-antioxidant balance (PAB) in in vitro fertilization (IVF)-conceived mice in comparison to naturally conceived offspring.
Methods: Mice (6?8 weeks) were divided into two groups (IVF-conceived and naturally conceived) matched by sex, age, weight, and litter size. A 1-mL blood sample was taken and the sera were separated. The oxidant-antioxidant balance was evaluated using a fast and reliable PAB assay. The results were expressed as mean±standard deviation.
Results: The mean PAB values (HK units) in the IVF-conceived and naturally conceived groups were 59.70±22.30 and 54.70±18.22, respectively (p=0.82).
Conclusion: Since free radicals contribute to several pathological conditions and antioxidants play an important protective role against oxidative stress, evaluating the oxidant-antioxidant balance is very important. Although the results of this study showed that the quality of the defense mechanism against free radicals was not significantly different between the IVF-conceived and naturally conceived mice, other parameters of metabolic dysfunction need to be measured.
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KEYWORD
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In vitro fertilization, Mice, Offsprings, Oxidative stress, Prooxidant-antioxidant balance
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